SWSAP1 is an ATPase component of the evolutionarily conserved Shu complex that plays a critical role in homologous recombination (HR) repair of DNA damage. As part of the SWS1-SWSAP1 heterodimer, SWSAP1 binds single-stranded DNA with exposed 5' ends and exhibits DNA-stimulated ATPase activity 1. The complex functions by binding and stabilizing RAD51 filaments, maintaining their structural integrity, and enhancing RPA diffusion on ssDNA to promote strand exchange during HR 2. SWSAP1 interacts with RAD51 and RAD51 paralogs, and its depletion causes defects in homologous recombination repair 3. Beyond somatic DNA repair, SWSAP1 is essential for meiotic recombination, promoting RAD51 and DMC1 assembly on early meiotic intermediates 4, and mutations in SWSAP1 cause premature ovarian insufficiency through impaired interhomolog homologous recombination 5. Cancer-associated variants in SWSAP1 alter Shu complex formation and stability, making SWSAP1 a potential therapeutic target for cancer treatment 2. SWSAP1 knockout cells show sensitivity to PARP and APE1 inhibition, suggesting relevance for cancer therapeutics 2.