C16orf95 is a chromosome 16 open reading frame with emerging roles in neurodegenerative disease and developmental pathways. Functionally, C16orf95 represents a novel genetic locus associated with phosphorylated tau (pTau) levels in cerebrospinal fluid, a core Alzheimer's disease biomarker 1. The gene shares genetic etiology with brain ventricular volume and glymphatic clearance activity, suggesting involvement in cerebrospinal fluid dynamics and brain waste clearance mechanisms 12. C16orf95 variants are significantly associated with normal pressure hydrocephalus (NPH) risk, with risk alleles increasing disease susceptibility (OR 1.24-1.28) 34. The gene's association with blood-brain and blood-cerebrospinal fluid barrier function indicates involvement in maintaining barrier integrity critical for neurological homeostasis 3. Additionally, C16orf95 deletions in 16q24.2 are associated with autism spectrum disorder, intellectual disability, and congenital renal malformations, suggesting developmental roles beyond neurodegeneration 56. The gene also shows pleiotropic effects on lipid metabolism, associating with HDL cholesterol and triglyceride variations 7. Overall, C16orf95 appears multifunctional, linking tau pathology, cerebrospinal fluid dynamics, barrier function, and developmental processes.