CDC20B (cell division cycle 20B) is a vertebrate-specific protein that plays a crucial role in multiciliated cell (MCC) differentiation by regulating deuterosome-mediated centriole production. CDC20B serves as a specific marker for deuterosomal cells, which are precursor cells that bridge the differentiation pathway between club cells and multiciliated cells 12. The protein associates with deuterosomes and is essential for centriole release and subsequent cilia production in MCCs 3. Mechanistically, CDC20B interacts with PLK1 kinase and coordinates centriole disengagement through the protease Separase, adapting canonical cell cycle machinery for the specialized process of multiciliogenesis 3. CDC20B is located on chromosome 5.2 and hosts the miR-449abc cluster in its second intron, with both the gene and its intronic microRNA being regulated by histone acetylation-deacetylation mechanisms 45. The gene's expression is significantly reduced during allergic inflammation, contributing to decreased multiciliated cell populations and impaired mucociliary function 2. CDC20B's role extends beyond normal physiology, as its dysregulation has been implicated in various pathological conditions including viral infections and developmental toxicity 67.