CDC25B is a dual-specificity tyrosine/threonine protein phosphatase that functions as a critical regulator of cell cycle progression and mitotic entry 1. The enzyme directly dephosphorylates and activates cyclin-dependent kinases (CDKs), particularly CDK1 and CDK2, thereby promoting G2/M phase transition and mitotic progression 12. CDC25B appears to function as a 'mitotic starter' that triggers an auto-amplification loop before being degraded by the proteasome pathway in a CDK1-cyclin A-dependent manner 1. The protein is regulated through its interaction with 14-3-3 proteins within the subcortical maternal complex (SCMC), which stabilizes CDC25B and ensures proper maturation-promoting factor activation during early embryogenesis 3. CDC25B overexpression has been linked to multiple cancers, including colorectal cancer, gastric cancer, and retinoblastoma, where it correlates with poor tumor differentiation, invasion, and clinical outcomes 456. The protein's role in cancer has made it an attractive therapeutic target, with various inhibitors being developed to block its phosphatase activity 72. Additionally, CDC25B contributes to DNA damage response pathways and is connected to Aurora kinase signaling during mitosis 8.