CKS1B (CDC28 protein kinase regulatory subunit 1B) is a regulatory protein essential for cyclin-dependent kinase function and cell cycle progression 1. The protein binds to catalytic subunits of cyclin-dependent kinases and is required for their biological activity, playing a critical role in cell cycle regulation 1. CKS1B functions within the SCF ubiquitin ligase complex and cyclin-dependent protein kinase holoenzyme complex, regulating mitotic cell cycle progression 2. The gene is frequently amplified or overexpressed in human cancers, where it promotes tumor progression, metastasis, and therapeutic resistance 314. In multiple myeloma, CKS1B amplification at chromosome 1 is associated with poor prognosis and shorter progression-free survival after autologous stem cell transplantation 56. The protein enhances cancer cell proliferation, migration, and stemness properties while inhibiting apoptosis 4. CKS1B is transcriptionally regulated by FOXM1 in pancreatic cancer, establishing a signaling axis that drives carcinogenesis and chemoresistance 4. Beyond oncology, CKS1B regulates the proliferation-to-maturation transition in cardiomyocytes through the Hedgehog-GLI2-CKS1B cascade 7. Pan-cancer analyses demonstrate CKS1B overexpression across multiple tumor types, correlating with poor clinical outcomes and representing a potential therapeutic target 8.