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GeneE
10 sources retrieved · Most recent: April 2026 · Index updated 14 days ago
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CNOT6L
CCR4-NOT transcription complex subunit 6 like
Chromosome 4 · 4q21.1
NCBI Gene: 246175Ensembl: ENSG00000138767.14HGNC: HGNC:18042UniProt: B4E0K8
47PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
FUNCTIONAL ROLE
Highly ConstrainedTranscription Factor
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
nucleuscytoplasmcytosolprotein bindingpreeclampsiaalcohol drinkingtype 2 diabetes mellituspolycystic ovary syndrome
✦AI Summary

CNOT6L is a catalytic subunit of the CCR4-NOT complex with 3'-5' poly(A) exoribonuclease activity that functions as a major mRNA deadenylase 1. It catalyzes poly(A) tail shortening of target mRNAs, likely through interaction with 3'-UTR cis-elements, thereby regulating mRNA stability and expression levels across multiple physiological processes 2. CNOT6L mediates diverse cellular functions through deadenylation-dependent mRNA decay. In metabolic homeostasis, CNOT6L suppresses hepatokine expression (GDF15 and FGF21) to modulate food intake and energy expenditure; CNOT6L inhibition stabilizes these mRNAs and ameliorates diet-induced metabolic syndrome 3. In cardiac physiology, CNOT6L prevents heart failure progression by downregulating tenascin-C mRNA in cardiac fibroblasts, thereby suppressing fibrosis and inflammation 2. In skeletal muscle, CNOT6L regulates myogenesis through post-transcriptional repression of IL-8 4. CNOT6L promotes cell proliferation and survival while preventing cellular senescence 1, though elevated expression correlates with poor outcomes in type 2 diabetes and triple-negative breast cancer 56. CNOT6L expression is regulated by the E3 ubiquitin ligase RNF219, which targets it for proteasomal degradation 6. These findings establish CNOT6L as a critical regulator of gene expression with therapeutic potential in metabolic and cardiovascular diseases.

Sources cited
1
CNOT6L deadenylase activity suppresses GDF15 and FGF21 hepatokine mRNAs; CNOT6L inhibition increases these mRNAs and improves metabolic syndrome
PMID: 35385705
2
CNOT6L catalyzes deadenylation of tenascin-C mRNA through 3'-UTR interaction; CNOT6L deletion increases cardiac fibrosis and dysfunction
PMID: 40023604
3
RNF219 E3 ubiquitin ligase directly binds CNOT1 and ubiquitinates CNOT6L for proteasomal degradation; low RNF219 associates with poor prognosis in triple-negative breast cancer
PMID: 40598799
4
CNOT6L deadenylase subunit promotes cell proliferation and survival while preventing senescence; distinct from other CCR4-NOT subunits in regulating IGFBP5
PMID: 21233283
5
CNOT6L is highly expressed in type 2 diabetes and regulates insulin signaling, PPAR, and cAMP pathways; associated with poor prognosis
PMID: 39433858
6
CNOT6L directly deadenylates IL-8 mRNA and suppresses its expression; regulates skeletal muscle myogenesis
PMID: 26608607
7
CNOT6L catalytic domain structure shows nucleotides and neomycin inhibit deadenylase activity by competing for substrate and magnesium-binding sites
PMID: 27013054
8
CNOT6L shares structural similarity with other CCR4 deadenylase family members including nocturnin and PDE12
PMID: 30389976
Disease Associationsⓘ20
preeclampsiaOpen Targets
0.08Suggestive
alcohol drinkingOpen Targets
0.06Suggestive
type 2 diabetes mellitusOpen Targets
0.06Suggestive
polycystic ovary syndromeOpen Targets
0.06Suggestive
ocular hypotensionOpen Targets
0.04Suggestive
lipodystrophy, congenital generalized, type 5Open Targets
0.04Suggestive
PLIN1-related familial partial lipodystrophyOpen Targets
0.03Suggestive
PPARG-related familial partial lipodystrophyOpen Targets
0.03Suggestive
obesity due to melanocortin 4 receptor deficiencyOpen Targets
0.03Suggestive
strictureOpen Targets
0.02Suggestive
cataractOpen Targets
0.01Suggestive
Abnormal sperm morphologyOpen Targets
0.01Suggestive
acute myocardial infarctionOpen Targets
0.01Suggestive
COVID-19–associated multisystem inflammatory syndrome in adultsOpen Targets
0.01Suggestive
breast cancerOpen Targets
0.01Suggestive
squamous cell carcinomaOpen Targets
0.01Suggestive
tuberculosisOpen Targets
0.01Suggestive
neoplasmOpen Targets
0.01Suggestive
diabetes mellitusOpen Targets
0.00Suggestive
acute quadriplegic myopathyOpen Targets
0.00Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
DDX6Protein interaction100%MMP7Protein interaction100%CNOT3Protein interaction100%CNOT4Protein interaction100%CNOT9Protein interaction100%TOB1Protein interaction100%
Tissue Expression6 tissues
Bone Marrow
100%
Brain
80%
Lung
75%
Ovary
61%
Heart
50%
Liver
49%
Gene Interaction Network
Click a node to explore
CNOT6LDDX6MMP7CNOT3CNOT4CNOT9TOB1
PROTEIN STRUCTURE
Preparing viewer…
PDB3NGQ · 1.80 Å · X-ray
View on RCSB ↗
Constraintⓘ
LOEUFⓘ
0.17Highly Constrained
pLIⓘ
1.00Intolerant
Observed/Expected LoF0.07 [0.03–0.17]
RankingsWhere CNOT6L stands among ~20K protein-coding genes
  • #9,190of 20,598
    Most Researched47
  • #293of 17,882
    Most Constrained (LOEUF)0.17 · top 5%
Genes detectedCNOT6L
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure.
PMID: 35385705
Cell Metab · 2022
1.00
2
CNOT6L deadenylase suppresses cardiac remodeling in heart failure through downregulation of tenascin-C mRNA.
PMID: 40023604
J Pharmacol Exp Ther · 2025
0.90
3
The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity.
PMID: 40598799
FEBS Open Bio · 2025
0.80
4
The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not complex contribute to the prevention of cell death and senescence.
PMID: 21233283
Mol Biol Cell · 2011
0.70
5
CNOT6L regulates hepatokine expression.
PMID: 35523890
Nat Rev Endocrinol · 2022
0.60