CTBS (chitobiase) is a reducing-end exoglycosidase that catalyzes glycan degradation through hydrolysis of N,N'-diacetylchitobiose and higher chitin-oligosaccharides to monomeric GlcNAc by acting on reducing-end residues 123. During N-linked glycoprotein degradation, CTBS functions downstream of glycosylasparaginase to cleave single GlcNAc molecules uniquely from the reducing end of N,N'-diacetylchitobiose units, thereby completing protein deglycosylation. This enzyme localizes to lysosomes and participates in carbohydrate catabolic processes, demonstrating chitinase activity and oligosaccharide catabolism. CTBS represents a key component of the lysosomal hydrolytic machinery responsible for processing glycoproteins destined for degradation. The clinical relevance of CTBS dysfunction remains incompletely characterized in the provided literature; however, defects in glycan degradation pathways have potential implications for lysosomal storage disorders and metabolic disease. No disease-specific associations or detailed clinical significance data are available in the provided abstracts.