DDX1 (DEAD-box helicase 1) is an ATP-dependent RNA helicase that plays crucial roles in RNA metabolism and genome stability. The protein exhibits DNA/RNA helicase activity and participates in both R-loop formation and resolution, contributing to transcription regulation and DNA repair processes 1. DDX1 demonstrates exceptional tight binding affinity to ADP, three orders of magnitude stronger than ATP binding, which may require nucleotide exchange factors for enzyme recycling 2. The protein shows strong cooperativity between RNA and ATP binding, reflected in its ATP hydrolysis activity 2. DDX1 undergoes post-translational modification through EZH2-mediated methylation at lysine 234, which disrupts its interaction with splicing factors and affects RNA processing, particularly promoting exon 14 skipping in MATR3 3. In cancer biology, DDX1 is frequently co-amplified with MYCN in neuroblastoma, mapping within 400 kb of the MYCN locus 4. Recent genomic studies have identified DDX1 as a novel risk gene for Alzheimer's disease and related dementia through rare variant analysis 5. Additionally, DDX1 interacts with YTHDF2 and eIF3F to enhance protein synthesis in ovarian cancer cells, facilitating translation of m6A-modified mRNAs encoding microtubule-associated proteins 6.