DHX57 is a probable ATP-binding RNA helicase 1 with RNA binding and helicase activity. While its precise molecular function remains incompletely characterized, DHX57 is conserved with DExH-box helicase domains and has been identified as a putative homolog of the yeast translation initiation factor Ylr419wp 1, suggesting a potential role in translation initiation. Clinically, DHX57 emerges as a significant biomarker in male infertility. It was identified as one of seven key genes in a seventeen-gene signature predictive model for non-obstructive azoospermia (NOA), with an area under the curve >0.8 in both testing and validation datasets 2. Notably, DHX57 mRNA is downregulated in NOA testicular samples compared to controls 2. Additionally, DHX57 was identified as a hub gene in comprehensive transcriptomic analysis of NOA sperm 3, and its expression is regulated in the context of aluminum-induced cognitive impairment through zinc-mediated ROCK1 pathway modulation 4. Genetically, a SNP within DHX57 (2p22.1) shows cis-acting effects on cerebellar DHX57 expression levels and associates with epigenetic age acceleration, with gene sets showing overlap with Alzheimer's disease, age-related macular degeneration, and Parkinson's disease 5. DHX57 has also been identified in a complex fusion gene (DHX57:TMEM178:MAP4K3) in pediatric high-grade glioma 6, suggesting potential involvement in oncogenic processes.