DMRT3 is a transcription factor located on chromosome 9 that functions primarily in configuring spinal circuits controlling locomotor coordination. In vertebrates, DMRT3 is expressed in spinal cord interneurons and plays a critical role in developing coordinated limb movement networks 1. A specific missense mutation (Ser301STOP) in DMRT3 causes the 'gaited' phenotype in horses, enabling comfortable ambling and pacing gaits, with this allele showing high frequency in gaited breeds and harness racing horses 12. Beyond locomotion, DMRT3 participates in sexual differentiation through regulation of estrogen receptor 1 (ESR1) expression. In disorders of sex development (DSDs), mutations in DMRT3 interact with OAS3 to prevent ESR1 mRNA degradation, causing 46,XY sex reversal and hypospadias 3. Recent evidence indicates DMRT3 transcriptionally regulates the lncRNA OIP5-AS1, which promotes pyroptosis in bronchial epithelial cells during asthma by stabilizing YAP mRNA 4. Clinically, DMRT3 deletion is associated with 9p deletion syndrome, affecting 83% of individuals with this condition 56. A deletion of a DMRT3 enhancer element has been linked to spastic cerebral palsy pathogenesis through impaired forebrain development and gait coordination 7. Additionally, DMRT3 shows aberrant expression in multiple cancer types, correlating with poor prognosis and potential chemoresistance 8.