DNASE1 is a serum endocuclease secreted by exocrine and endocrine organs that plays critical roles in DNA catabolism and inflammation regulation 1. The enzyme preferentially cleaves protein-free DNA and is involved in apoptotic cell death processes 1. A primary function of DNASE1 is degrading neutrophil extracellular traps (NETs)—web-like structures composed of DNA fibers released by neutrophils during inflammation. Together with DNASE1L3, DNASE1 degrades intravascular NETs to prevent pathological thrombosis and organ damage 23. DNASE1 also binds G-actin and inhibits actin polymerization. Dysregulation of DNASE1 activity contributes to multiple disease states. Impaired NET clearance due to reduced DNASE1 function exacerbates atherosclerosis progression by promoting macrophage efferocytosis defects 4. DNASE1 treatment substantially attenuates acetaminophen-induced acute liver injury by reducing NET-mediated hepatocyte PANoptosis 5. Similarly, DNase1 administration diminishes myocardial ischemia-reperfusion injury and fibrotic vascular occlusions in chr16 thrombosis 32. Abnormal plasma nuclease activity, including altered DNASE1 levels, is associated with systemic lupus erythematosus and affects cell-free DNA fragmentation patterns 6. These findings establish DNASE1 as a key therapeutic target across multiple inflammatory and thrombotic conditions.