EEPD1 (endonuclease/exonuclease/phosphatase family domain containing 1) is a multifunctional protein with primary roles in DNA repair and metabolic regulation. As a DNase1 superfamily member, EEPD1 functions in oxidative damage repair and stressed replication fork restart 12. The protein localizes to the plasma membrane through N-terminal myristoylation and palmitoylation, particularly in response to high cholesterol levels 32. Mechanistically, EEPD1 regulates cholesterol homeostasis by functioning as an LXR target gene that enhances ABCA1-dependent cholesterol efflux from macrophages 3. Additionally, EEPD1 promotes actin polymerization and cell migration through SRC-RAC1 signaling pathways 2, and activates PKA-mediated lipolysis and thermogenesis in adipose tissue following myristoylation 4. Diseases involving EEPD1 dysregulation include obesity (reduced EEPD1 expression accelerates development), atherosclerosis (elevated EEPD1 promotes endothelial apoptosis via ERK phosphorylation), and NASH exacerbated by obstructive sleep apnea (CIH-mediated autophagic degradation of EEPD1 increases hepatic DNA damage) 456. MicroRNA-320b suppression of EEPD1 impairs macrophage cholesterol efflux and atherosclerosis progression 7. Clinically, pharmacological restoration of EEPD1 with Retigabine dihydrochloride effectively mitigates both obesity and NASH progression, suggesting EEPD1 as a promising therapeutic target for metabolic and cardiovascular diseases 46.