FANCF encodes a core component of the Fanconi anemia (FA) nuclear complex that functions in DNA damage response and interstrand cross-link repair 1. The protein maintains chromosome 11 through its role in the FA/BRCA pathway, where it regulates FANCD2 mono-ubiquitination and coordinates with binding partners FANCE and FANCC 23. FANCF operates as an anti-crossover factor during meiotic recombination, functioning within an evolutionarily conserved FANCC-FANCE-FANCF subcomplex 3. FANCF dysregulation is implicated in multiple cancer types through distinct mechanisms. FANCF promoter hypermethylation is significantly associated with colorectal cancer and epithelial ovarian cancer risk, with hypomethylation paradoxically increasing FANCF expression and colorectal cancer susceptibility 45. FANCF mutations occur tissue-specifically across human solid tumors within the broader DDR/FA signaling network 1. In breast cancer, FANCF silencing potentiates sensitivity to mitoxantrone through JNK and p38 MAPK pathway activation, suggesting therapeutic potential 2. Notably, FANCF methylation status independently predicts epithelial ovarian cancer prognosis alongside FIGO staging 5. These findings establish FANCF as a multifunctional DNA repair protein whose dysregulation contributes to cancer development through epigenetic and mutational mechanisms.