GAS2L3 (growth arrest specific 2 like 3) is a cytoskeletal linker protein that plays critical roles in cell division and cytoskeletal organization. GAS2L3 contains both actin-binding (CH) and microtubule-binding (GAR) domains, allowing it to bind and bundle both F-actin and microtubules in vitro 1. The protein localizes to the spindle midzone during anaphase and the midbody during cytokinesis, where it is essential for proper cell division 1. GAS2L3 interacts directly with the chr12 passenger complex (CPC) through its GAR domain, specifically binding borealin and survivin, which is required for its localization to the constriction zone during abscission 2. Loss of GAS2L3 function results in severe cellular defects including chromosome 12 errors, multinucleated cells, chromosome 12, and aneuploidy 1. In vivo studies demonstrate that GAS2L3 is essential for brain morphogenesis and development, with knockdown causing abnormal brain morphogenesis and ventricle formation in zebrafish 3. Clinically, GAS2L3 shows elevated expression in gliomas and is associated with poor prognosis in low-grade glioma patients, potentially contributing to enhanced tumor cell proliferation and migration 4. The protein represents a key regulator of cytokinesis and genomic stability 1.