GOLPH3 is a phosphatidylinositol-4-phosphate-binding protein that localizes to the trans-Golgi network and serves as a cargo adaptor for COPI-coated vesicles 1. Its primary function involves maintaining Golgi architecture and regulating vesicular trafficking between Golgi compartments and the plasma membrane 2. Mechanistically, GOLPH3 links Golgi membranes to the cytoskeleton, facilitating membrane dynamics required for vesicle budding and Golgi ribbon formation 1. It maintains proper Golgi localization of glycosylation enzymes and the LYSET-GNPT complex, essential for mannose-6-phosphate tagging and lysosomal enzyme trafficking 3. Clinically, GOLPH3 is frequently amplified in multiple solid tumors including breast cancer, glioblastoma, colorectal cancer, and lung cancer, where overexpression correlates with poor prognosis and tumor metastasis 24. Its oncogenic activity involves enhanced mTOR signaling; Drosophila studies demonstrate GOLPH3 physically interacts with mTOR pathway components (Tctp and 14-3-3 proteins) and regulates Golgi localization of Rheb, promoting mTORC1 activation and organ growth 5. Additionally, GOLPH3 influences cancer phenotypes through controlling exosome content and loading of oncogenic molecules 4, and participates in DNA damage response and genomic stability maintenance 2. These multifaceted roles make GOLPH3 a promising therapeutic target for cancer intervention.