IFT54 is a component of the intraflagellar transport (IFT) complex B involved in bidirectional trafficking within cilia 1. During anterograde movement, IFT54 binds active kinesin-II along the ciliary microtubule axoneme while transporting the inactive dynein-2 complex for retrograde movement 1. IFT54 contains a divergent N-terminal Calponin Homology domain important for its molecular function 1. Beyond canonical ciliogenesis, IFT54 functions as a negative regulator of microtubule stability through MAP4 control, contributing to epithelial polarity in non-ciliary contexts 2. IFT54 is essential for immune synapse assembly, where it functions within an IFT20 complex regulating TCR recycling and phosphotyrosine signaling at the immune synapse 3. During cardiogenesis, IFT54 modulates the Hippo pathway effector YAP1, restricting proepicardium and myocardium formation 4. SMYD3-mediated regulation of IFT54 trafficking is critical for cilia formation and maintenance 5. Clinically, mutations in IFT54 cause Senior-Loken syndrome, a ciliopathy characterized by nephronophthisis and retinal degeneration 2. IFT54's transition fiber protein interactions are required for ciliary entry of assembled IFT particles 6.