KHSRP (KH-type splicing regulatory protein) is a multifunctional RNA-binding protein located on chromosome 19.3 1 that regulates gene expression through multiple mechanisms. Primary functions include mediating alternative splicing by activating splicing through intronic enhancer sequences 1 and interacting with splicing factor SF3B1 to promote pre-mRNA splicing 2. KHSRP also recognizes m6A-modified mRNAs and stabilizes their expression; it binds m6A methylated transcripts to prevent degradation by exoribonuclease XRN2 3. Additionally, KHSRP facilitates miRNA maturation in an m6A-dependent manner 4. Clinically, KHSRP dysregulation is implicated in multiple malignancies. In pancreatic ductal adenocarcinoma, elevated KHSRP stabilizes FAK pathway mRNAs (MET, ITGAV, ITGB1) to promote tumor progression and poor survival, with KHSRP PROTAC targeting showing therapeutic promise 5. KHSRP promotes cisplatin resistance in bladder cancer through the CLASP2/MAPRE1 axis 6, enhances glucose metabolism reprogramming in liver cancer via the FBL/KHSRP-PFKFB4 axis 7, and mediates radiotherapy resistance in bone-metastatic prostate cancer 3. Conversely, KHSRP protects against acute liver failure by facilitating proper pre-mRNA splicing 2, and functions as a tumor suppressor in colorectal cancer by promoting miR-320a maturation 4. In diabetic cataracts, KHSRP operates within the METTL3/TUG1/p38MAPK signaling axis to regulate cell proliferation and apoptosis 8.