KIF14 is a microtubule motor protein that plays critical roles in cell division, cytokinesis, and tumor progression. The protein functions as a key regulator of cytokinesis by targeting to the central spindle and midbody through interactions with PRC1 and CIT 1. KIF14 is significantly overexpressed across multiple cancer types, including cholangiocarcinoma, gastric cancer, glioblastoma, triple-negative breast cancer, retinoblastoma, lung adenocarcinoma, medulloblastoma, and colorectal cancer 23456789. High KIF14 expression consistently correlates with poor prognosis, increased metastasis, and chemotherapy resistance 238. Mechanistically, KIF14 promotes tumor progression through multiple pathways including AKT signaling 34, NF-κB activation via G3BP1/YBX1 complex binding 2, and regulation of immune infiltration 75. KIF14 silencing inhibits cell proliferation, migration, invasion, and induces apoptosis, while promoting G2/M cell cycle arrest 48. The protein serves as an independent prognostic biomarker and potential therapeutic target across multiple malignancies 98.