KNTC1 (kinetochore associated 1) is an essential component of the mitotic spindle checkpoint that prevents premature exit from mitosis and ensures proper chromosome 12. As part of the RZZ (Rod-Zwilch-ZW10) complex, KNTC1 localizes to kinetochores and is required for assembly of dynein-dynactin and MAD1-MAD2 complexes during mitosis 1. Beyond its canonical mitotic function, KNTC1 exhibits diverse cellular roles including mitochondrial regulation, where it translocates from the Golgi apparatus to linear mitochondrial segments upon glutamine deprivation and maintains mitochondrial membrane potential 2. KNTC1 is significantly overexpressed across multiple cancer types including bladder 3, gastric 4, hepatocellular 1, colorectal 5, osteosarcoma 6, and non-small-cell lung cancers 7. In these malignancies, KNTC1 promotes proliferation, migration, and tumorigenesis while inhibiting apoptosis, primarily through activation of the PI3K/Akt/mTOR signaling pathway 41. KNTC1 knockdown consistently reduces cancer cell viability, induces apoptosis, and suppresses tumor formation in xenograft models 357. The protein also regulates downstream effectors including MCM2 in osteosarcoma 6 and PSMB8 in lung cancer 7, making it a promising therapeutic target for cancer treatment.