LAMA5 encodes laminin subunit alpha-5, a critical extracellular matrix component mediating cell attachment, migration, and tissue organization during development 1. The protein functions through integrin-mediated signaling and plays essential roles in skeletal system morphogenesis and basement membrane assembly 2. In cardiac organoids, endothelial-derived LAMA5 regulates expression of mature sarcomeric proteins and contractility 1. LAMA5 promotes endothelial cell migration, proliferation, and angiogenesis via PI3K-AKT-MTOR signaling pathways; notably, LAMA5 expression is significantly decreased in preeclampsia placentas, correlating with vascular dysfunction 3. In pancreatic cancer, LAMA5 mediates cancer-associated fibroblast-induced acinar-to-ductal cell transdifferentiation through an integrin-α4/STAT3 axis, contributing to PDAC initiation 4. Clinically, LAMA5 mutations cause congenital myasthenic syndromes and nephrotic syndrome affecting neuromuscular junction and kidney function 5. In ovarian cancer, elevated LAMA5 expression predicts poor overall survival and promotes cell proliferation and migration via Notch signaling pathway activation 6. Genome-wide association studies identified LAMA5 in cell adhesion pathways associated with nicotine dependence 7, with functional studies demonstrating nicotine suppresses LAMA5 expression and abolishes nicotine-induced cell migration in LAMA5-knockout cells.