LATS1 (large tumor suppressor kinase 1) is a serine/threonine kinase and core component of the Hippo signaling pathway that functions as a critical tumor suppressor 1. LATS1 operates within a kinase cascade where MST1/2 phosphorylates and activates LATS1 in complex with its regulatory protein MOB1, which then phosphorylates and inactivates the oncoproteins YAP1 and TAZ 1. This phosphorylation prevents YAP1 nuclear translocation, inhibiting expression of genes promoting cell proliferation and migration while suppressing apoptosis 1. Beyond canonical Hippo signaling, LATS1 maintains chr6 stability through regulation of mitotic progression and G1 tetraploidy checkpoints, controls G2/M transition by downregulating CDK1 activity, and affects cytokinesis through negative modulation of actin polymerization 2. LATS1 expression and activity are dynamically regulated by multiple mechanisms: m6A methylation by METTL3 reduces LATS1 mRNA stability in breast cancer 3, while oxidative stress activates LATS1 through MOB1 acetylation 2. LATS1 degradation via WWP1-mediated ubiquitination promotes metastasis in esophageal cancer 4. Recently, LATS1 was identified as a NLRP3 inflammasome activator, phosphorylating NLRP3 to facilitate its interaction with NEK7 5. Loss or reduced LATS1 expression is associated with tumorigenesis, making it a therapeutic target across multiple cancer types.