AJUBA is a LIM domain scaffolding protein that regulates multiple cellular processes through assembly of diverse protein complexes 1. The protein contains nuclear export and import sequences, enabling cytoplasmic-nuclear shuttling to coordinate cell adhesion, motility, mitosis, and gene expression 1. AJUBA functions as a negative regulator of the Hippo signaling pathway, promoting cell proliferation and survival through YAP activation 2. In gastric cancer, AJUBA overexpression enhances glucose metabolism and mitochondrial function via the YAP-GLUT1/Bcl-xL axis, contributing to chemoresistance and tumor progression 2. AJUBA plays context-dependent roles in cancer, functioning as both tumor promoter and suppressor in epithelial malignancies 3. The gene is frequently mutated in esophageal squamous cell carcinoma (7% of cases), where it contributes to Hippo pathway dysregulation 4. AJUBA mutations are also found in head and neck squamous cell carcinomas, particularly in laryngeal tumors, suggesting involvement in WNT pathway alterations 5. In placental development, MYBL2-mediated AJUBA regulation promotes extravillous trophoblast differentiation and migration, with dysregulation contributing to recurrent spontaneous abortion 6. Clinically, AJUBA expression levels correlate with tumor progression and patient prognosis across various cancer types 1.