LIPN (lipase family member N) is a specialized enzyme that plays a critical role in keratinocyte differentiation and skin barrier formation. The protein contains two distinct domains: an alpha/beta hydrolase fold and an abhydrolase-associated lipase region, with the consensus sequence of an active lipase 1. LIPN functions in the terminal stages of keratinocyte differentiation, specifically in lipid metabolism of the most differentiated epidermal layers 1. The enzyme localizes within putative lamellar bodies alongside other lipoxygenases, suggesting involvement in the formation of the corneocyte lipid envelope (CLE), a critical structure for skin barrier function 2. Mutations in LIPN cause autosomal recessive congenital ichthyosis type 8 (ARCI8), a rare skin cornification disorder characterized by generalized scaling and varying degrees of erythema 13. In clinical populations, LIPN mutations account for approximately 2% of ARCI cases, making it one of the less common genetic causes within this heterogeneous disorder 34. The identification of LIPN mutations is facilitated by mRNA analysis using hair follicle samples, which provides a minimally invasive diagnostic approach for genetic counseling and patient management 1.