LMNB1 encodes lamin B1, an intermediate filament protein that assembles into the nuclear lamina, a fibrous meshwork on the nucleoplasmic side of the inner nuclear membrane 12. As a major structural component of the nuclear envelope, LMNB1 provides a framework bridging the nuclear envelope and chr5, playing critical roles in nuclear assembly, chr5 organization, and nuclear membrane dynamics 12. The structural integrity of the lamina is tightly controlled during the cell cycle, with disintegration during prophase and reformation during telophase 12. LMNB1 expression is significantly reduced in senescent cells, serving as a marker of cellular senescence 34. In cancer biology, elevated LMNB1 expression correlates with poor prognosis across multiple tumor types and is associated with enhanced DNA repair capacity and Th2 immune cell infiltration 5. In glioma, LMNB1 knockdown inhibits cell proliferation, induces apoptosis, and reduces migration through PI3K/Akt pathway suppression 6. Clinically, LMNB1 mutations and structural variants cause autosomal dominant adult-onset demyelinating leukodystrophy (ADLD), with duplications causing classical ADLD and noncoding deletions causing atypical ADLD 78. B-type lamins including LMNB1 are essential for neuronal migration in developing brain 8, and LMNB1 dysregulation particularly affects forebrain function in ADLD pathogenesis 7.