MAPRE1 (EB1) is a plus-end tracking protein (+TIP) that binds microtubule plus-ends and regulates microtubule cytoskeleton dynamics 1. It recruits other +TIP proteins through recognition of conserved Ser-X-Leu-Pro motifs and promotes cytoplasmic microtubule nucleation and elongation 2. MAPRE1 undergoes liquid-liquid phase separation to form membraneless condensates that enrich tubulin dimers and coordinate microtubule dynamics 1. During mitosis, MAPRE1 stabilizes astral microtubules and facilitates their cortical attachment for spindle positioning; this function is regulated by TIP60-dependent crotonylation at Lys66 3. MAPRE1 also interacts with CENP-R through phase separation to guide chromosome 20 during metaphase 4. Additionally, MAPRE1 regulates non-centrosomal minus-end microtubule organization by recruiting CAMSAP2 to the Golgi apparatus, supporting polarized cell movement and autophagosome transport 5. Disease relevance includes associations with polycystic ovary syndrome 6, ulcerative colitis 7, nasopharyngeal carcinoma 8, and bladder cancer cisplatin resistance 9. Clinically, plasma MAPRE1 elevation shows promise as a biomarker for early-stage colorectal cancer and adenomas 10.