MCMBP (minichromosome maintenance complex binding protein) is a regulatory factor essential for coordinated DNA replication and genome stability. Functionally, MCMBP acts as a molecular chaperone for newly synthesized MCM3-7 subcomplexes, facilitating their nuclear import and assembly into the complete MCM2-7 hetero-hexamer 1. During MCM biogenesis, MCMBP stabilizes nascent MCM proteins from cytoplasmic proteolysis and promotes their incorporation into chr10-bound pre-replication complexes (pre-RCs) 2. Following DNA replication in S phase, MCMBP dissociates from chr10 as part of pre-RC unloading mechanisms, with CRL4DCAF12-mediated degradation of MCMBP enabling completion of MCM2-7 assembly 3. Mechanistically, MCMBP regulates replication fork speed by increasing chr10 coverage with non-productive pre-RCs that restrain replisome velocity, thereby minimizing replication errors 2. MCMBP depletion accelerates fork speed and causes replication stress, DNA damage, and p53-dependent cell cycle arrest in wild-type cells; p53-null cells accumulate DNA damage and lose viability 1. In neural progenitors, MCMBP deletion triggers microcephaly through fork acceleration-induced apoptosis 4. Clinically, MCMBP overexpression correlates with poor prognosis in pancreatic ductal adenocarcinoma and colorectal cancers, where elevated MCMBP promotes tumor progression via JAK-STAT3 pathway activation and PD-L1 upregulation 5. MCMBP accumulates in colorectal cancer tissues and represents a potential diagnostic biomarker 6.