MMP16 (matrix metallopeptidase 16) is a membrane-type matrix metalloproteinase that functions as a zinc-containing endopeptidase involved in extracellular matrix remodeling and tissue development 1. As a member of the MT-MMP family, MMP16 degrades various ECM components including collagen type III and fibronectin, and activates progelatinase A 1. The enzyme plays crucial roles in multiple developmental processes, particularly lung alveolarization, where it shows low expression during early developmental stages but increases significantly during the alveolar stage 2. MMP16 is also essential for ovarian function, with its expression increased by hCG administration during the periovulatory period across species including rats, macaques, and humans 3. Disease relevance includes associations with bronchopulmonary dysplasia in preterm infants, where specific MMP16 polymorphisms provide protective effects 2, and involvement in osteosarcopenia as a risk factor 4. Additionally, MMP16 contributes to vascular calcification and coronary artery disease 5, and is implicated in various cancers including hepatocellular carcinoma where it promotes malignancy 6. Clinical significance extends to cleft lip/palate susceptibility 7 and age-related cataract progression 8.