NAA60 (N-alpha-acetyltransferase 60, NatF) is a Golgi-localized acetyltransferase that catalyzes N-terminal acetylation of transmembrane proteins with cytosol-facing N-termini 12. It displays activity toward diverse N-terminal sequences including Met-Lys, Met-Val, Met-Ala, and Met-Met residues 31. NAA60 also possesses lysine acetyltransferase activity on histone H4 4, though in vivo significance requires clarification 5. The protein contains a GNAT domain and amphipathic helix critical for Golgi localization and catalytic function 4. Biologically, NAA60 participates in chromosome 16 during anaphase 3 and regulates interferon-α signaling during viral infection 6. It acetylates the phosphate importer SLC20A2, maintaining surface protein levels and extracellular phosphate uptake 2. Additionally, NAA60 facilitates platinum drug uptake by acetylating LRRC8A/D anion channel subunits 7. Clinically, biallelic NAA60 variants cause autosomal recessive primary familial brain calcification (PFBC), a neurodegenerative disorder featuring bilateral basal ganglia calcifications with progressive movement, cognitive, and psychiatric symptoms 289. Loss-of-function variants disrupt NAA60 protein localization and degrade the protein, impairing acetylation capacity and affecting phosphate homeostasis and brain calcification-associated proteins 10.