NAA80 (N-alpha-acetyltransferase 80) is the dedicated N-terminal acetyltransferase for actin, catalyzing post-translational acetylation of processed beta- and gamma-actin N-termini 1. NAA80 preferentially acetylates actin with acidic N-terminal sequences (Asp-Asp-Asp and Glu-Glu-Glu motifs) 1, operating on monomeric actin-profilin complexes rather than filamentous actin 2. This acetylation regulates actin filament dynamics by modulating both depolymerization and elongation rates, particularly affecting formin-driven elongation 1. Functionally, NAA80 activity critically controls cytoskeletal assembly and cell motility; NAA80-knockout cells show increased filopodia/lamellipodia formation, elevated F-actin levels, and accelerated migration 1. Beyond cytoskeletal organization, NAA80-mediated actin acetylation is essential for Golgi apparatus structural integrity 3 and normal hearing through proper inner ear development 4. Biallelic NAA80 mutations cause auroneurodental syndrome, characterized by sensorineural hearing loss, developmental delay, muscle weakness, and craniofacial dysmorphisms due to impaired actin acetylation 5. Recent evidence suggests NAA80 may contribute to attention-deficit/hyperactivity disorder pathogenesis through altered excitatory-inhibitory neuron function 6.