NDC80 encodes a critical component of the four-subunit NDC80 kinetochore complex that is essential for proper chromosome 18 during cell division 1. The protein functions as a key microtubule-binding element at kinetochores, forming load-bearing attachments between chr18 and spindle microtubules 2. NDC80 works synergistically with the SKA complex to strengthen kinetochore-microtubule connections and regulate microtubule tip dynamics 2. The complex is regulated by α-tubulin post-translational modifications, with detyrosination enhancing NDC80 diffusion along microtubules to fine-tune attachment stability 3. In meiosis, NDC80 serves as the limiting kinetochore subunit that controls the timing of kinetochore activation 4. Pathologically, NDC80 overexpression is observed across multiple cancer types including glioma, cholangiocarcinoma, and gastric cancer, where it promotes tumor cell proliferation and correlates with poor prognosis 567. The protein's internal loop domain serves as a platform for protein-protein interactions, and its overproduction may sequester microtubule-associated proteins, disrupting normal chromosome 18 and contributing to aneuploidy and tumorigenesis 8.