NEK3 (NIMA-related kinase 3) is a serine/threonine protein kinase that functions in cell cycle regulation and cytoskeletal dynamics. As a member of the NIMA family of kinases, NEK3 is expressed preferentially in mitotically active tissues including testis, ovary, and brain 1. NEK3 regulates intracellular membrane fusion through phosphorylation of SNAP29 at serine 105, directing its membrane association and affecting focal adhesion formation and Golgi structure 2. In prolactin signaling, NEK3 acts as a key mediator of breast cancer cell motility by phosphorylating Vav2 and paxillin, thereby activating the small GTPase Rac1 and promoting cytoskeletal reorganization 34. NEK3 overexpression correlates with poor prognosis in gastric cancer, promoting tumor malignancy through enhanced migration and proliferation 5. Recent evidence demonstrates NEK3's role as a suppressor of cuproptosis; NEK3 depletion enhances copper-dependent cell death and synergizes with immunomodulatory effects by promoting M1 macrophage polarization in cervical cancer 6. These findings establish NEK3 as an important oncogenic kinase and potential therapeutic target across multiple cancer types.