NID1 encodes nidogen-1, a sulfated glycoprotein that serves as a critical structural component of basement membranes throughout the body 1. As a key extracellular matrix protein, nidogen-1 functions as a molecular bridge linking laminin and collagen IV networks within basement membranes, providing structural integrity and organization 21. The protein plays essential roles in cell-extracellular matrix interactions and tissue homeostasis 3. In vascular biology, nidogen-2 (a related family member) maintains smooth muscle cell contractile phenotype through Jagged1-Notch3 signaling, preventing pathological neointima formation 4. NID1 has significant clinical relevance in multiple disease contexts. Human cytomegalovirus specifically targets NID1 for degradation, potentially facilitating viral dissemination while causing developmental defects 5. In oncology, elevated NID1 expression correlates with worse outcomes in KMT2A-rearranged pediatric acute myeloid leukemia and associates with leukemic stem cell metabolic phenotypes 6. Additionally, NID1 upregulation contributes to liver fibrosis progression in NASH through activation of IL-6/JAK/STAT3 signaling and macrophage polarization 7. The protein also serves as a biomarker for right ventricular dysfunction in pulmonary hypertension 8.