NIN (ninein) is a centrosomal protein essential for microtubule organization in epithelial cells. Its primary function is positioning and anchoring the microtubule minus-end at the centrosome 12. NIN recruits the gamma-tubulin ring complex to facilitate centrosome-templated microtubule nucleation and serves as a centrosome maturation factor 31. At the molecular level, NIN localizes to centriolar subdistal appendages and the pericentriolar material, where it mediates centriole organization and microtubule anchoring at the mother centriole 2. Functionally, NIN overexpression suppresses microtubule release without perturbing nucleation or elongation 1, indicating a specific role in stabilizing anchored microtubules. Clinically, NIN mutations are associated with Seckel syndrome 7, a rare autosomal recessive dwarfism disorder. The centrosomal defects caused by NIN dysfunction likely impair cell division and neurogenesis, explaining the growth retardation and microcephaly characteristic of this condition. Understanding NIN's role in centrosome biology provides insights into developmental disorders and potential targets for conditions involving centrosomal dysfunction.