NOL4L (nucleolar protein 4 like) is a nuclear protein localized to the nucleoplasm with protein-binding capacity 1. Primary function involves chr20 regulation through interaction with CHD4, a chr20 remodeling factor, where NOL4L cooperates to suppress Notch signaling pathway genes 2. NOL4L is expressed across multiple tissues during vertebrate development, including brain, spinal cord, and hematopoietic cells 1. In cancer biology, NOL4L promotes tumor progression through multiple mechanisms. In ovarian cancer, NOL4L enhances cell proliferation and metastasis by activating the PI3K/AKT signaling pathway, with elevated expression correlating with poor prognosis 3. In neuroblastoma, NOL4L functions downstream of multiple regulatory axes: the miR-432-5p/NOL4L axis 4, miR-362-5p/NOL4L axis 5, miR-29/NOL4L axis 6, and miR-676-3p/NOL4L axis 7. These pathways involve circular RNAs and long non-coding RNAs acting as competing endogenous RNAs that sequester microRNAs from NOL4L, thereby promoting tumor cell proliferation, migration, invasion, and glycolytic metabolism. Clinically, NOL4L gene fusions with RUNX1 and PAX5 occur in acute myeloid leukemia and acute lymphoblastic leukemia, respectively 1, 8, positioning NOL4L as a potential therapeutic target for multiple malignancies.