NPAP1 (Nuclear Pore Associated Protein 1), located on chromosome 15-q13, is a paternally imprinted gene encoding a 1156 amino acid protein that localizes to the nuclear pore complex (NPC) 1. The protein contains six nuclear localization sequences and shows sequence similarity to the nucleoporin POM121 1. Immunocytochemical and structured illumination microscopy studies demonstrate that NPAP1 colocalizes with NPCs and nuclear lamins at the inner nuclear envelope 1. NPAP1 is evolutionarily conserved in primates and subject to strong positive Darwinian selection, suggesting important functions in primate-specific biology 23. The gene originated from retrotransposition of POM121 and subsequently underwent genomic imprinting 3. While the precise molecular function remains incompletely characterized, NPAP1 has been implicated in neurobehavioral development, with expression variations in placental tissue associated with altered neonatal neurological outcomes 4. Additionally, NPAP1 mutations have been identified as part of a prognostic biomarker panel in neuroendocrine neoplasms, where mutations correlated with significantly poorer patient survival 5. The gene's role in Prader-Willi syndrome pathogenesis remains unclear, though deletions encompassing NPAP1 have been observed in atypical PWS cases 6.