NUBP2 is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery essential for extramitochondrial Fe/S cluster biogenesis 1. The protein forms a heterotetramer with NUBP1 to create a Fe/S scaffold complex that mediates de novo assembly of Fe/S clusters and their transfer to target apoproteins 2. NUBP2 belongs to a conserved nucleotide-binding protein family containing ATP/GTP-binding motifs and shares distinctive NUBP/MRP sequence motifs with prokaryotic counterparts 2. The protein exhibits cytosolic and nuclear localization patterns dependent on N-terminal modifications 3. NUBP2 plays critical developmental roles, as demonstrated by mouse studies showing that neural crest-specific deletion causes severe craniofacial defects and increased apoptosis, leading to prenatal lethality 4. In cancer contexts, NUBP2 shows altered expression patterns associated with poor prognosis in hepatocellular carcinoma 5 and promotes tumor progression in colorectal cancer through GSK3β signaling modulation 6. The protein can be targeted by metal-based therapeutics that disrupt Fe/S cluster biogenesis at sub-cytotoxic concentrations 1. Additionally, alternative splicing of NUBP2 is regulated by metformin treatment, contributing to anti-cancer effects 7.