RAB35 is a small GTPase that serves as a master regulator of membrane trafficking with diverse cellular functions. As a member of the Rab family, RAB35 cycles between inactive GDP-bound and active GTP-bound states to recruit specific downstream effectors 1. The protein plays essential roles in endocytic recycling, functioning as a rate-limiting regulator of the fast recycling pathway back to the plasma membrane 2. During cytokinesis, RAB35 is required for intercellular bridge stability and abscission, working alongside RAB11 to orchestrate the completion of cell division 3. RAB35 serves as a central regulator of phosphoinositides and F-actin both on endosomes and at the plasma membrane, explaining its involvement in diverse processes including phagocytosis, cell migration, and neurite outgrowth 2. In neurons, RAB35 coordinates synaptic vesicle trafficking and turnover, with its signaling network being critical for synaptic protein homeostasis 4. The protein has emerged as clinically significant, with activating mutations conferring oncogenic properties in cancer 5. Additionally, RAB35 can be phosphorylated by LRRK2 kinase, linking it to Parkinson's disease pathways through lysosomal tubulation processes 6. Mutations in RAB35 regulatory proteins are associated with neurologic and neurodegenerative diseases 4.