RAD54L is a multifunctional ATPase that functions as a molecular motor in homologous recombination (HR), a major DNA double-strand break repair pathway 123. The protein guides RAD51 ssDNA along donor dsDNA, converting homology search from a diffusion-based to a motor-guided mechanism 1. RAD54L plays essential roles in RAD51-mediated synaptic complex formation and dissociates RAD51 from nucleoprotein filaments post-recombination 1. Additionally, RAD54L functions in replication fork remodeling, restraining fork progression under replication stress and suppressing ssDNA gap formation through both BRCA1/2- and 53BP1-dependent pathways 4. RAD54L expression is significantly elevated in multiple cancers including hepatocellular carcinoma, ovarian cancer, bladder cancer, and multiple myeloma, correlating with poor prognosis and aggressive phenotypes 5678. RAD54L knockdown inhibits cancer cell proliferation and enhances gemcitabine sensitivity in hepatocellular carcinoma 5. In multiple myeloma, RAD54L silencing induces cell cycle arrest and apoptosis 8. RAD54L genetic variants associate with meningioma development and histopathological characteristics 910. These findings identify RAD54L as a potential therapeutic target and prognostic biomarker across multiple malignancies.