RAP2B is a small GTPase belonging to the Ras oncogene family that cycles between GDP-bound inactive and GTP-bound active states 1. The protein plays crucial roles in cytoskeletal organization and cell migration, with its cellular localization regulated by S-palmitoylation at C-terminal cysteine residues C176/C177, which is essential for plasma membrane targeting 2. RAP2B functions as a p53 target gene and regulates cytoskeletal dynamics by inhibiting cell spreading through disruption of actin dynamics 3. In cancer contexts, RAP2B is consistently upregulated across multiple tumor types including gliomas, breast cancer, colorectal cancer, and suprarenal epithelioma 4526. The protein promotes oncogenic processes through multiple signaling pathways: it enhances cell proliferation, migration, and invasion via calcium-mediated ERK1/2 activation in breast cancer 5, activates MMP2/MMP9 expression through ERK signaling in gliomas 7, and regulates NF-κB/MMP pathways 4. RAP2B's palmitoylation is regulated by EGFR/PI3K signaling and ABHD17a depalmitoylating enzyme, making it a potential therapeutic target for metastatic cancers 2. Higher RAP2B expression correlates with poorer survival in low-grade glioma patients 7.