RFC2 (replication factor C subunit 2) is a critical component of the replication factor C complex that plays essential roles in DNA replication and repair processes. As part of the RFC complex, RFC2 functions in ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA templates, facilitating DNA polymerase delta and epsilon activity during DNA synthesis 1. The protein contributes to leading strand-specific PCNA loading through the CHTF18-RFC2/5 complex, which is crucial for maintaining genome stability and DNA replication processivity 1. RFC2 has significant clinical relevance across multiple cancer types. It is upregulated in colorectal cancer, where it promotes tumor progression through activation of aerobic glycolysis and the MET/PI3K/AKT/mTOR pathway 2. In glioblastoma, RFC2 expression correlates with poor patient survival, and its downregulation enhances chemotherapy sensitivity 3. The gene also shows oncogenic properties in cervical cancer, promoting cell proliferation, migration, and invasion 4. Additionally, RFC2 may contribute to Williams syndrome pathogenicity, as knockout zebrafish models demonstrate developmental abnormalities including small head and brain, jaw defects, and vascular problems 5. RFC2 expression is regulated by various microRNAs including miR-744 and miR-4749-5p, suggesting potential therapeutic targeting opportunities 63.