SLC35B3 encodes a 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter localized to the Golgi membrane that mediates PAPS transport from the cytosol into the Golgi lumen, enabling sulfation reactions within this compartment 1. As a key component of the sulfation machinery, SLC35B3 expression is upregulated by the aryl hydrocarbon receptor in response to the microbial metabolite indole-3-acetic acid, enhancing intestinal mucin sulfation and maintaining barrier homeostasis 2. Functionally, SLC35B3 is involved in critical developmental processes, with genome-wide association studies identifying SLC35B3 as significantly associated with cleft palate susceptibility 3. The gene also associates with glucose effectiveness and type 2 diabetes risk in Mexican American populations 4. Additionally, SLC35B3 expression is altered in chemotherapy-resistant ovarian cancer tissues 5, and copy number variations at the SLC35B3 locus show nominal associations with non-small cell lung cancer development 6. The gene lies within a region susceptible to microdeletion on chromosome 6.1p24.3, where loss of SLC35B3 and neighboring genes contributes to developmental abnormalities including intellectual disability and cardiac defects 7. These findings establish SLC35B3 as a multifunctional sulfation transporter with roles in intestinal homeostasis, craniofacial development, metabolic regulation, and cancer biology.