SLC35F3 (solute carrier family 35 member F3) is a transmembrane transporter that mediates both thiamine and choline transport across cell membranes 12. The protein functions as a thiamine transporter with demonstrated uptake activity when expressed in bacterial systems 1. As a choline transporter, SLC35F3 exhibits a KM value of 12-50 µM, aligning with physiological plasma choline concentrations, and shows sensitivity to inhibition by hemicholinium-3, decynium-22, and verapamil 2. The transporter plays a crucial role in carbohydrate metabolism through its thiamine transport function, as thiamine is essential for glycolysis and the tricarboxylic acid cycle 3. Genetic variants in SLC35F3 have significant clinical implications, with rs10910387 polymorphisms associated with hypertension and blood pressure regulation 1, and interactions with dietary carbohydrate intake affecting metabolic syndrome risk 3. Additionally, SLC35F3 expression has been identified as a prognostic biomarker in cancer, with elevated expression correlating with poor overall survival in lung adenocarcinoma and serving as a potential therapeutic target 45. The gene also shows associations with mood stabilizer treatment response in bipolar disorder 6.