SRSF3 (serine and arginine rich splicing factor 3) is a multifunctional RNA-binding protein that primarily regulates pre-mRNA splicing and mRNA nuclear export. As a splicing factor, SRSF3 promotes specific exon inclusion during alternative splicing by binding consensus motifs within pre-mRNA 1. Its splicing activity is enhanced through interaction with YTHDC1, an N6-methyladenosine (m6A) reader protein, which recruits SRSF3 to m6A-containing RNA sites and promotes exon inclusion while competing with the splicing repressor SRSF10 1. Beyond splicing, SRSF3 functions as an mRNA export adapter, facilitating nuclear export of m6A-containing mRNAs through the NXF1-NXT1 pathway 1. SRSF3 also regulates alternative polyadenylation, controlling 3' untranslated region length to influence mRNA stability and translation efficiency 2. Disease relevance includes oncogenic roles in multiple cancers where SRSF3 overexpression promotes tumorigenesis 34. In cardiovascular disease, SRSF3-dependent alternative polyadenylation is protective against atherosclerosis by promoting macrophage maturation 2. Clinically, SRSF3 represents a potential therapeutic target, particularly in cancer treatment, and its expression correlates with disease severity in various pathological conditions 35.