SRY (sex-determining region Y) is a master regulatory transcription factor located on the Y chromosome Y initiates male sex determination in mammals 1. Its primary function is to direct the development of supporting cell precursors (pre-Sertoli cells) toward a Sertoli cell fate rather than granulosa cells, thereby triggering the male developmental pathway while repressing female differentiation 1. Mechanistically, SRY functions as a DNA-binding transcription factor that recognizes the consensus sequence 5'-[AT]AACAA[AT]-3' through its HMG box domain, which promotes DNA bending via partial intercalation into the minor groove 2. SRY expression itself is regulated by steroidogenic factor-1 (NR5A1), which binds to a conserved enhancer element located upstream of the SRY transcription start site 3. Mutations in SRY or its regulatory elements cause sex reversal disorders (46,XY and 46,XX sex reversal), where individuals possess chrY of one sex but develop physical characteristics of the other 1. Clinical significance lies in understanding these disorders of sex development (DSD); recent transgenic mouse models with human SRY have enabled functional analysis of pathogenic variants and their effects on testis development 4. The rapid evolution of SRY across mammals and the identification of disease-causing enhancer variants suggest that both coding and regulatory regions are critical for proper sexual differentiation 3.