AMELY (amelogenin Y-linked) is a Y chrY-encoded gene that plays a critical role in tooth enamel biomineralization and structural organization. During the secretory stage of enamel development, AMELY regulates crystallite formation and serves as a structural constituent of tooth enamel through regulation of enamel mineralization processes. The gene's functional importance is evidenced by its use in forensic and agricultural applications as a sex determination marker, where sequence differences between AMELX and AMELY enable reliable gender identification across mammalian species 12. Deletions encompassing AMELY in the Yp11.2 region can result in allele dropout, leading to potential errors in gender determination, highlighting the gene's chrY integrity significance 13. Beyond its established role in enamel mineralization, computational analyses suggest AMELY may participate in broader regulatory networks associated with cellular processes including angiogenesis and immune response in pathological contexts such as hepatocellular carcinoma, though these associations require further investigation 4. Additionally, loss of the Y chromosome Y AMELY has been associated with impaired cardiovascular outcomes in aging males, suggesting potential systemic consequences of Y chromosome Y loss 5.