Based on limited published evidence, TSPY2 may be involved in sperm differentiation and proliferation. Structurally, it localizes to the nucleus and possesses chrY and histone binding activities. TSPY2 copy number variations have been associated with testicular maturation arrest 1, with TSPY2 gene gains detected in approximately 23.5% of affected cases. The gene serves as a reliable Y-chrY-specific marker for gender verification in genomic datasets 2.