WT1 is a transcription factor that plays critical roles in both normal development and cancer pathogenesis 1. As a transcription factor, WT1 regulates cellular development and survival by recognizing and binding to specific DNA sequences, controlling the expression of numerous target genes 1. WT1 is essential for urogenital system development, particularly in podocyte development within the kidney, where it regulates key genes including BMPER, PAX2, MAGI2, MYH9, and NPHS1 that control WNT signaling, actin filament organization, and cell junction assembly 2. The gene exhibits functional complexity with different isoforms: those lacking the KTS motif act as transcription factors, while KTS-containing isoforms may bind mRNA and participate in RNA metabolism 1. WT1 has dual roles in cancer, functioning both as a tumor suppressor and oncogene depending on context 13. Congenital mutations cause Wilms tumor, Denys-Drash syndrome, and Frasier syndrome 14, while somatic mutations occur in various leukemias 15. In renal carcinoma, WT1 inhibits cell proliferation by upregulating IL-24 and inducing G2/M cell cycle arrest 6. WT1 also shows promise as a therapeutic target, being highly immunogenic and ranking as the top cancer antigen for immunotherapy applications 3.