ZBED3 (zinc finger BED-type containing 3) is a transcriptional regulator that plays critical roles in metabolic homeostasis and cancer biology. The protein functions as a positive regulator of the canonical Wnt/β-catenin signaling pathway by stabilizing cytoplasmic β-catenin and promoting transcription of Wnt target genes 12. ZBED3 promotes hepatic steatosis by interacting with polypyrimidine tract-binding protein 1 (PTBP1) to regulate SREBP1c mRNA stability and alternative splicing, contributing to fatty acid synthesis 3. Elevated ZBED3 expression is associated with metabolic syndrome, correlating positively with adiposity markers, glucose parameters, and inflammatory markers 4. In cancer contexts, ZBED3 acts as an oncogenic factor, promoting hepatocellular carcinoma cell proliferation through the Wnt/β-catenin pathway 5 and lung cancer cell growth via β-catenin regulation and PCNA expression 1. The protein's BED finger domain is essential for its regulatory functions, particularly in cancer cell proliferation 1. Additionally, ZBED3 participates in the subcortical maternal complex and plays a role in early embryonic development, with variants potentially causing female infertility through early embryo arrest 6.