ZNF516 (zinc finger protein 516) is a transcriptional regulator with tumor suppressor properties that functions primarily through chr18 modification complexes. The protein acts as a transcriptional repressor by physically associating with the CtBP/LSD1/CoREST complex to regulate genes involved in cell proliferation and motility, including EGFR 1. ZNF516 binds directly to gene promoters, such as ABCC5, to repress their transcriptional activation 2. Loss of ZNF516 expression is strongly associated with cervical cancer progression, showing inverse correlation with lesion grade and high-risk HPV infection 3. The ZNF516 promoter undergoes frequent hypermethylation in cervical neoplasia, serving as a biomarker with 90% sensitivity and specificity for cancer detection 4. In breast cancer, low ZNF516 expression correlates with advanced pathological staging and poor survival, while the ZNF516-CtBP/LSD1/CoREST complex suppresses cancer cell proliferation, invasion, and metastasis 1. Additionally, ZNF516 is regulated by miR-371-5p and plays roles in trophoblast cell function during placental development 5. The protein also contributes to drug sensitivity in lung adenocarcinoma by repressing ABCC5 expression 2.