AFAP1 (actin filament associated protein 1) is a cytoskeletal regulatory protein that cross-links actin filaments into network and bundle structures, modulates actin filament integrity, and induces lamellipodia formation. AFAP1 functions as an adapter molecule linking proteins such as SRC and PKC to the actin cytoskeleton, with particular roles in focal adhesion regulation and signal transduction. Mechanistically, AFAP1 regulates cell-matrix adhesions and migration, processes critical for both normal cellular function and pathological conditions. The protein operates at the intersection of cytoskeletal dynamics and cell signaling, positioning it as a key regulator of cellular motility and invasive characteristics 1. Disease relevance extends across multiple organ systems. AFAP1 has been identified as a genetic risk locus for primary open-angle glaucoma (POAG) in genome-wide association studies 2, and more recently as an aortic stenosis-specific genetic risk factor independent of coronary artery disease 3. Additionally, AFAP1's antisense RNA, AFAP1-AS1, is aberrantly expressed in numerous malignancies including lung, gastric, hepatocellular, ovarian, and breast cancers, where it promotes epithelial-mesenchymal transition, migration, invasion, and chemotherapy resistance 456. Clinically, AFAP1 expression patterns and AFAP1-AS1 dysregulation correlate with poor prognosis and tumor progression, positioning these molecules as potential biomarkers and therapeutic targets for cancer diagnosis and treatment 61.